Thermo Fisher Scientific FLIP Polyclonal Antibody
FLIP 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot과 Immunocytochemistry에 적합합니다. Mouse 시료에 반응하며, 비결합형 액상 형태로 제공됩니다. 세포 사멸 경로 연구 및 FLIP 단백질 검출에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to amino acids 449–465 of mouse FLIPL/CASHa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153370 |
Product Specific Information
- In Western blot applications, this antibody detects a band at approximately 55 kDa.
- A suggested positive control is NIH-3T3 cell lysate.
- PA5-19881 can be used with blocking peptide PEP-0013.
Target Information
FLIP (CFLAR) is an apoptosis regulator protein that serves as a key link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6-mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC), blocking further recruitment and processing of caspase-8. Full-length and shorter isoforms have been shown to either induce apoptosis or reduce TNFRSF-triggered apoptosis. FLIP lacks enzymatic (caspase) activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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