Thermo Fisher Scientific EpCAM/CD326 (Epithelial Marker) Monoclonal Antibody (rMOC-31)
EpCAM/CD326 단백질을 표적하는 마우스 모노클로날 항체로, 인간 시료에 반응하며 다양한 면역분석(WB, IHC, ICC, Flow 등)에 사용 가능. 단백질 A/G 정제, 액상 형태로 제공되며 4°C 보관. 상피세포 표지 및 암 연구에 적합.
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Thermo Fisher Scientific EpCAM/CD326 (Epithelial Marker) Monoclonal Antibody (rMOC-31)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | rMOC-31 |
| Immunogen | Recombinant full-length human EpCAM protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Rat, Dog, or Cat.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic cell adhesion molecule and influences cell cycle, proliferation, and metabolism via induction of c-myc, cyclin A, and cyclin E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with increased epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors.
Ep-CAM is found on the baso-lateral surface of most simple epithelia and many carcinoma types, and can help distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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