Thermo Fisher Scientific Tn Antigen Monoclonal Antibody (Tn 218)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

Tn 218

Immunogen

Asialo ovine submaxillary mucin (AOSM).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.20 mg/mL

Storage buffer

PBS, pH 7.2

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1961080

Product Specific Information

MA1-90544 stains Tn antigen in epithelial carcinoma, pancreatic, colon, stomach and breast.

A suggested positive control for this product is human gastrointestinal tumor.

The antibody is supplied as concentrated tissue culture supernatant in PBS with 0.05% azide.

Target Information

Aberrant glycosylation is a hallmark of cancer cells. The blood group precursors T (Thomsen-Friedenreich) and Tn epitopes are shielded in healthy and benign-diseased tissues but uncovered in approximately 90% of carcinomas. T and Tn glycoproteins are specific, autoimmunogenic pancarcinoma antigens. These antigens may also be found in neoplastic blood cells. Fundamental, chemical and physical aspects of these glycoproteins of primary carcinomas are discussed first. Tn and T epitopes are cell and tissue adhesion molecules, essential in invasion by and metastasis of carcinoma which includes adherent and proliferative phases. Immunohistochemical studies of the extent of Tn antigen expression in primamry breast carcinoma demonstrate its highly significant correlation with clinical pathological tumor stages, and hence its value as a reliable prognisticator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.