Thermo Fisher Scientific 6-His Tag Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000 – 1:5,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:200 – 1:500

ELISA

• Tested Dilution: 1:1,000 – 1:10,000

Product Specifications

Product Specific Information

Goats were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Note: This antibody does not detect all N-terminal 6-His tagged proteins.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal of recombinant proteins to enable purification by immobilized metal affinity chromatography.

Epitope tagging allows detection of recombinant proteins by fusing a known epitope, facilitating identification when no specific antibody exists. His-tagged proteins are purified via metal chelate chromatography due to the strong and selective affinity of histidine clusters for metal ions such as Ni²⁺.

His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.