Thermo Fisher Scientific 6-His Tag Polyclonal Antibody, HRP
6x His 태그 단백질 검출용 HRP 결합 폴리클로날 항체. Western blot, ICC/IF, ELISA 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 신뢰성 제공. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000 – 1:5,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:200 – 1:500
ELISA
- Tested Dilution: 1:1,000 – 1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.05% Pro-Clean 400 |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Goats were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Note: This antibody does not detect all N-terminal 6-His tagged proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal of recombinant proteins to enable purification by immobilized metal affinity chromatography.
Epitope tagging allows detection of recombinant proteins by fusing a known epitope, facilitating identification when no specific antibody exists. His-tagged proteins are purified via metal chelate chromatography due to the strong and selective affinity of histidine clusters for metal ions such as Ni²⁺.
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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