Thermo Fisher Scientific 6-His Tag Polyclonal Antibody
6x His 태그 단백질 검출용 폴리클로날 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용 가능. 염소 유래, 항원 친화 크로마토그래피 정제. His 태그 단백질의 정제 및 검출 연구에 적합.
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Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Goats were immunized with 6-His (HHHHHH) conjugated to KLH. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 6.8 to 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
This antibody does not detect all N-terminal 6-His tagged proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
His tags are commonly expressed at either the N- or C-terminal regions of recombinant proteins to enable isolation or purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of proteins lacking specific antibodies by fusing a known epitope sequence.
His-tagged proteins can be purified efficiently using Ni²⁺-NTA columns due to the strong and selective binding affinity between histidine residues and nickel ions.
His tag-specific antibodies are widely used for detection or co-immunoprecipitation of tagged recombinant proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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