Thermo Fisher Scientific Glu-Glu Tag Polyclonal Antibody
Glu-Glu 태그 단백질 검출용 토끼 폴리클로날 항체. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 사용 가능. EYMPME 서열 기반 합성 펩타이드 면역원으로 제작. 항원 친화 크로마토그래피로 정제된 고순도 항체.
- 카탈로그번호
- A190111A
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide representing amino acid residues 314–319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Rabbits were immunized with a synthetic peptide representing amino acid residues 314–319 (EYMPME) of the middle T antigen of mouse polyomavirus conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Target Information
The Glu-Glu tag, also known as the EYMPME sequence, is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with other amino acids. It is typically incorporated into the N-terminus of proteins through subcloning into expression vectors such as the pFASTBAC vector. This tag is particularly useful in recombinant protein expression and purification, facilitating detection and isolation of tagged proteins via specific antibodies that recognize the Glu-Glu sequence. The Glu-Glu tag can be used in various systems, including baculovirus expression in insect cells, and enables efficient studies of protein-protein interactions, localization, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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