Thermo Fisher Scientific Glu-Glu Tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000 - 1:30,000

-

Immunocytochemistry (ICC/IF)

1:100 - 1:400

-

ELISA (ELISA)

1:1,000 - 1:30,000

-

Immunoprecipitation (IP)

1 - 4 µg/mg lysate

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 6.8 to 7.4

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Rabbits were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Target Information

The Glu-Glu tag, also known as the EYMPME sequence, is a short peptide epitope tag consisting of two glutamic acid (Glu) residues interspersed with various amino acids. It is typically incorporated into the N-terminus of proteins through subcloning into vectors such as the pFASTBAC vector. This tag is particularly useful in recombinant protein expression and purification processes. It facilitates the detection and isolation of tagged proteins via specific antibodies that recognize the Glu-Glu sequence. The Glu-Glu tag can be utilized in various systems, including baculovirus expression in insect cells, and provides efficient means for studying protein-protein interactions, localization, and function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.