Thermo Fisher Scientific 6-His Tag Polyclonal Antibody, Biotin

Thermo Fisher Scientific 6-His Tag Polyclonal Antibody, Biotin

Applications and Tested Dilution

• Western Blot (WB): 1:10,000 – 1:100,000
• ELISA: 1:10,000 – 1:100,000

Product Specifications

Product Specific Information

Rabbits were immunized with 6-His (HHHHHH) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
This antibody does not detect all N-terminal 6-His tagged proteins.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed as a tag at either the N- or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography.

Epitope tagging enables detection of recombinant proteins for which no antibody is available.
His-tagged proteins are purified via metal chelate chromatography due to the strong and selective affinity of histidine clusters for Ni²⁺ ions.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.