Thermo Fisher Scientific StAR (Steroidogenic Acute Regulator) Monoclonal Antibody (STAR, 2140)
인간 StAR 단백질을 표적으로 하는 단클론 항체로, 면역조직화학(IHC) 및 펩타이드 어레이에 사용 가능. 단백질 A/G 정제, PBS 완충액 내 0.05% BSA 포함. 스테로이드 생성 관련 연구용으로 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | STAR, 2140 |
| Immunogen | Recombinant fragment (around aa 39–108) of human STAR protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed):
Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane.
The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone.
StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissues.
Abnormalities in StAR gene expression are implicated in autosomal Lipoid Congenital Adrenal Hyperplasia (LCAH), resulting in defects in pregnenolone and cortisol synthesis.
The mechanism of cholesterol binding to StAR has yet to be elucidated.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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배송 정보
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