Thermo Fisher Scientific Glucocorticoid Receptor beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 18 publications 18 publications

Immunohistochemistry (IHC)

-

View 9 publications 9 publications

Immunocytochemistry (ICC/IF)

1:1,500

View 3 publications 3 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

-

Gel Shift (GS)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Pig, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2155928

Product Specific Information

PA3-514 detects glucocorticoid receptor (GR) beta from human cells.

PA3-514 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~90 kDa protein representing GR beta from HeLa cell lysate. Immunocytochemical staining of GR beta in HeLa cells with PA3-514 results in nuclear staining.

PA3-514 immunizing peptide corresponds to amino acid residues 728-742 from human GR beta. This peptide (Cat. # PEP-222) is available for use in neutralization and control experiments.

Target Information

Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex`s subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR beta might function as a dominant negative inhibitor of GR alpha activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.