Thermo Fisher Scientific FGF10 Polyclonal Antibody, PeproTech
Human FGF10 단백질을 인식하는 염소 유래 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 세포 성장 및 조직 형성 연구용으로 사용됩니다.
- 카탈로그번호
- 500-P151G-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FGF10 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human FGF-10 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2929490 |
Product Specific Information
AA Sequence of recombinant protein:
MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human FGF-10. Anti-Human FGF-10-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-10 matrix.
Sandwich ELISA:
To detect Human FGF-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-10 (500-P151GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human FGF-10.
Western Blot:
To detect Human FGF-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-10 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
This protein exhibits mitogenic activity for keratinizing epidermal cells but essentially no activity for fibroblasts, similar to the biological activity of FGF7.
Studies of the mouse homolog suggest that this gene is required for embryonic epidermal morphogenesis, including brain development, lung morphogenesis, and limb bud formation.
FGF10 is also implicated as a primary factor in the process of wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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