Thermo Fisher Scientific FGF10 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human FGF-10. Anti-Human FGF-10-specific antibody was purified by affinity chromatography employing an immobilized Human FGF-10 matrix.

Sandwich ELISA:
To detect Human FGF-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human FGF-10 (500-P151GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human FGF-10.

Western Blot:
To detect Human FGF-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-10 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

FGF10 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
This protein exhibits mitogenic activity for keratinizing epidermal cells but essentially no activity for fibroblasts, similar to the biological activity of FGF7.
Studies of the mouse homolog suggest that this gene is required for embryonic epidermal morphogenesis, including brain development, lung morphogenesis, and limb bud formation.
FGF10 is also implicated as a primary factor in the process of wound healing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.