Thermo Fisher Scientific FGF10 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human FGF-10. Anti-Human FGF-10-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
To detect Human FGF-10 by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. When used with PeproTech Polyclonal Anti-Human FGF-10 (500-P151G) as a capture antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Human FGF-10.

Western Blot:
For detection of hFGF-10 by Western blot, use 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for recombinant hFGF-10 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P151GBT-1MG is provided as 2 × 500 µg.

Target Information

FGF10 is a member of the fibroblast growth factor (FGF) family, which exhibits broad mitogenic and cell survival activities. It plays roles in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF10 shows mitogenic activity for keratinizing epidermal cells but minimal activity for fibroblasts, similar to FGF7. The mouse homolog is essential for embryonic epidermal morphogenesis, including brain and lung development and limb bud formation. FGF10 is also implicated as a key factor in wound healing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.