Thermo Fisher Scientific FGF17 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human FGF-17 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FGF17,uniProtId:O60258-1,ncbiNodeId:9606,antigenRange:23-216,antigenLength:216,antigenImageFileName:500-P152BT-25UG_FGF17_O60258-1_Rabbit.svg,antigenImageFileNamePDP:500-P152BT-25UG_FGF17_O60258-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929501

Product Specific Information

AA Sequence of recombinant protein: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-17. Anti-Human FGF-17-specific antibody was purified by affinity chromatography and then biotinylated.

Direct ELISA: To detect Human FGF-17 by direct ELISA (using 100 µL/well) a concentration of approximately 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 2000-4000 pg/mL of Recombinant Human FGF-17.

Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-17 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P152BT-1 mg will be provided as 2 x 500 µg

Target Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was shown to be prominently expressed in the cerebellum and cortex. The mouse homolog of this gene was localized to specific sites in the midline structures of the forebrain, the midbrain-hindbrain junction, developing skeleton and developing arteries, which suggests a role in central nervous system, bone and vascular development. This gene was referred to as FGF-13 in reference 2, however, its amino acid sequence and chromosomal localization are identical to FGF17.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.