Thermo Fisher Scientific FGF17 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-17. Anti-Human FGF-17-specific antibody was purified by affinity chromatography and then biotinylated.

Direct ELISA:
To detect Human FGF-17 by direct ELISA (using 100 µL/well), a concentration of approximately 1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 2000–4000 pg/mL of Recombinant Human FGF-17.

Western Blot:
To detect hFGF-17 by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hFGF-17 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P152BT-1 mg will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is prominently expressed in the cerebellum and cortex. The mouse homolog is localized to specific sites in the forebrain midline structures, midbrain-hindbrain junction, developing skeleton, and arteries, suggesting roles in CNS, bone, and vascular development. This gene was previously referred to as FGF-13, but its amino acid sequence and chromosomal localization are identical to FGF17.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.