Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), PE-Cyanine7, eBioscience

Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 6 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Brilliant UV 615
• eFluor 450
• eFluor 660
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio440c recognizes Siglec-H, a protein found exclusively on plasmacytoid dendritic cells (pDC) or type I IFN-producing cells (IPC) in naive mice.
Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and respond rapidly to viral infections.

Siglec-H is a transmembrane protein of the Ig superfamily similar to CD33, capable of binding sialic acid but lacking a cytoplasmic ITIM domain. It associates with DAP12 for signal transduction.

The eBio440c antibody inhibits pDC function (reduces IFN-α secretion in response to CpG).
Some mouse strains (e.g., SJL) have higher percentages of pDCs compared to C57BL/6.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes
Recommended usage: ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration is recommended for optimal performance.

Light Sensitivity: Tandem dye sensitive to photo-induced oxidation. Protect from light.
Fixation: Samples can be stored using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Excitation / Emission: 488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Siglec-H is expressed exclusively on plasmacytoid dendritic cells (pDCs) or type I IFN-producing cells (IPC) in naive mice.
It belongs to the Ig superfamily and binds sialic acid but lacks a cytoplasmic ITIM domain.
Associates with DAP12 for signal transduction.
The eBio440c antibody inhibits pDC function and IFN-α secretion.
Mouse strain differences observed (SJL > C57BL/6 in pDC percentage).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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