Thermo Fisher Scientific CD33 Monoclonal Antibody (WM-53 (WM53)), PE-Cyanine7, eBioscience
인간 CD33 항원을 표적하는 WM-53 단일클론 항체로, PE-Cyanine7 형광 표지된 유세포 분석용 항체입니다. 단핵구, 과립구 등 골수계 세포 표지에 적합하며, 광민감성이 있어 암실 보관이 필요합니다. 연구용으로만 사용 가능합니다.
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- 25-0338-4x (2개 옵션)
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD33 Monoclonal Antibody (WM-53 (WM53)), PE-Cyanine7, eBioscience
Applications
Immunocytochemistry (ICC/IF)
- Tested Dilution: Not specified
- View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- View 22 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM-53 (WM53) |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1907381 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 496
- Brilliant UV 661
- Brilliant Violet 605
- Brilliant Violet 711
- FITC
- NovaFluor Blue 690
- NovaFluor Red 710
- NovaFluor Yellow 570
- NovaFluor Yellow 660
- NovaFluor Yellow 755
- PE
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The WM-53 monoclonal antibody reacts with human CD33 (GP67, P67), a 67 kDa type I transmembrane glycoprotein in the Siglec family. CD33 is expressed in hematopoietic cells such as monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.
Applications Reported:
Suitable for flow cytometric analysis.
Applications Tested:
Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test in 100 µL final volume. Cell count: 10⁵–10⁸ cells/test (empirically determined).
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.
Excitation / Emission:
488–561 nm / 775 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD33 is a transmembrane protein of the Siglec (sialic acid-binding immunoglobulin-like lectin) family containing ITIM motifs that recruit SHP-1 and SHP-2 phosphatases. It mediates inhibitory signaling and receptor endocytosis. CD33 is expressed on myelomonocytic lineage cells, granulocyte and macrophage precursors, and peripheral monocytes, but not on pluripotent stem cells. It serves as a marker to distinguish myelogenous leukemia cells and is a therapeutic target for acute myeloid leukemia. Diseases associated with CD33 dysfunction include gallbladder lymphoma and extracutaneous mastocytoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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