Thermo Fisher Scientific Phospho-Btk (Tyr223) Recombinant Rabbit Monoclonal Antibody (BtkY223-B4), FITC
FITC 형광 표지된 인산화 Btk (Tyr223) 특이 재조합 토끼 단일클론 항체로, 인간 및 생쥐 시료에 반응. Flow Cytometry에 최적화된 5 µL/10⁶ 세포 희석 사용. 높은 특이성과 로트 간 일관성 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Phospho-Btk (Tyr223) Recombinant Rabbit Monoclonal Antibody (BtkY223-B4), FITC
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BtkY223-B4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896860 |
추가 포맷
- Unconjugated: MA5-36923
- APC: MA5-36926
- PE: MA5-36924
- Request custom conjugation available
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily. Mutations in the BTK gene are associated with severe developmental blocks in B-cell ontogeny and immunodeficiency disorders.
BTK interacts with toll-like receptor (TLR) family members such as TLR4, 6, 8, and 9, which are crucial for innate and adaptive immunity. It also interacts with proteins involved in TLR4 signaling, including MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting BTK’s role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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