Thermo Fisher Scientific Phospho-Btk (Tyr223) Recombinant Rabbit Monoclonal Antibody (BtkY223-B4), PE
PE 형광 표지된 Phospho-Btk (Tyr223) 항체로 인간 및 생쥐 시료에 적합. 재조합 토끼 단클론 항체로 높은 특이성과 재현성을 제공. Protein A/G로 정제되어 안정적이며, Flow Cytometry에 최적화. 4°C에서 보관.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BtkY223-B4 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896859 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily. Mutations in the BTK gene are linked to severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders.
BTK interacts with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9, which are critical in innate and adaptive immunity. It also interacts with key proteins in TLR4 signal transduction, including MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting a role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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