Thermo Fisher Scientific Phospho-Estrogen Receptor alpha (Ser106) Polyclonal Antibody
Phospho-Estrogen Receptor alpha (Ser106) 특이적 폴리클로날 항체. Western blot, IHC, ICC/IF에 적합. Human, Mouse, Rat 반응성. Rabbit IgG 기반, Affinity purified. PBS/glycerol buffer, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Suggested Positive Control |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | MCF-7 cells |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:100 | Human breast carcinoma |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | MCF-7 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine106 (S-P-S(p)-P-L) derived from Human Estrogen Receptor-alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554177 |
Product Specific Information
- Suggested positive control for Western blot: MCF-7 cells
- Suggested positive control for IHC: Human breast carcinoma
- Suggested positive control for ICC/IF: MCF-7 cells
Target Information
Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, upon binding to estrogen, undergoes a conformational change enabling dimerization and binding to estrogen response elements (ERE) in DNA. This binding allows ER to regulate gene transcription through coactivator or corepressor recruitment.
Two main forms exist — ERα (ESR1) and ERβ (ESR2) — encoded by separate genes. Alternative splicing produces at least four ERα isoforms (66, 53, 47, and 35 kDa). Estrogen receptors are expressed widely, crucial for sexual development, reproductive function, and bone maintenance. They are implicated in diseases such as breast cancer, endometrial cancer, and osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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