Thermo Fisher Scientific Phospho-Estrogen Receptor alpha (Ser104) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:100

Product Specifications

Product Specific Information

• Suggested positive control for Western blot: MCF-7 cells
• Suggested positive control for IHC: Human breast carcinoma

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, induces a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through recruitment of coactivator or corepressor proteins.

There are two forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2). At least four estrogen receptor-alpha isoforms are known to exist due to alternative RNA splicing (Isoform 1: 66 kDa, Isoform 2: 53 kDa, Isoform 3: 47 kDa, Isoform 4: 35 kDa). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play roles in tissues such as bone and are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음)