Thermo Fisher Scientific NFkB p100 Monoclonal Antibody (2F10A7)
인간 NFkB p100 단백질을 인식하는 마우스 단클론 항체로, Western blot, Flow cytometry, ELISA에 사용 가능. 정제된 재조합 단백질을 면역원으로 제작되었으며, Protein G로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F10A7 |
| Immunogen | Purified recombinant fragment of human NFKB2 (aa: 712–900) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898496 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA encoding an alternative DNA-binding subunit of NFkB has been isolated. It is synthesized as a protein expressed in various cell types and, like p105, undergoes cleavage to generate its NFkB subunit, in this case, a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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