Thermo Fisher Scientific NEDD8 Monoclonal Antibody (5B8)
NEDD8 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, ELISA에 적합합니다. 인간 시료 반응성이 있으며, 단백질 G로 정제된 액상 항체입니다. 세포주기 조절 연구 및 유비퀴틴 경로 분석에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B8 |
| Immunogen | Purified recombinant fragment of human NEDD8 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898492 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
NEDD8 is a ubiquitin-like protein that plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires activation by the E1 complex (UBE1C-APPBP1) and linkage to the E2 enzyme (UBE2M). Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, promoting polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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