Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), eFluor 450, eBioscience
인간 CD34 항원을 인식하는 단클론 항체로, eFluor 450 형광표지로 결합되어 유세포분석에 최적화됨. 조혈모세포 및 혈관내피세포 표지에 사용 가능. 405 nm 레이저에서 여기되어 445 nm에서 방출. 연구용으로만 사용.
- 카탈로그번호
- 48-0349-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 2 Publications |
| Flow Cytometry (Flow) | 5 µL (1 µg)/test | 53 Publications |
| Miscellaneous PubMed (Misc) | - | 2 Publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4H11 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10734946 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family that includes endoglycan and podocalyxin. These are single-pass transmembrane proteins with heavily glycosylated extracellular mucin domains.
CD34 was first identified on hematopoietic progenitors and is widely used as a marker to isolate cells capable of hematopoietic engraftment. It is also expressed on mesenchymal stem cells, tumor cell lines, and vascular endothelia in adults.
Epitopes of CD34 are divided into three classes (I, II, III) based on enzymatic sensitivity. The 4H11 antibody belongs to class III, reacting with a protein epitope.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (1 µg) per test in 100 µL final volume.
Laser Compatibility: Violet laser (405 nm excitation, 445 nm emission)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on many stem cell populations. It serves as a stem cell marker, involved in receptor-mediated endocytosis and regulation of adhesion, differentiation, and proliferation of hematopoietic progenitors.
CD34 expression may represent a specific developmental state of hematopoietic cells with altered adhesion and differentiation properties. It may mediate attachment to bone marrow matrix or stromal cells and act as a scaffold for glycan binding to selectins.
The intracellular domain is phosphorylated by protein kinase C, suggesting a role in signal transduction.
Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.