Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), FITC, eBioscience
Mouse, Rat TNF alpha 인식하는 중화용 단클론 항체로 FITC 형광 표지되어 있음. Flow cytometry, ELISA, neutralization 실험에 적합. 0.5 mg/mL 농도, PBS buffer에 0.09% sodium azide 포함. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 11-7423-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), FITC, eBioscience
Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 3 publications |
| ELISA | - | View 1 publication |
| Neutralization | - | View 2 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TN3-19.12 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465427 |
Available Formats
Product Specific Information
The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit TNF alpha, but not with human TNF alpha. It is a neutralizing antibody. Mouse TNF alpha (17 kDa) is produced by macrophages, monocytes, neutrophils, CD4+ T cells, and NK cells. A 26 kDa membrane-bound form also exists. TNF alpha plays a key role in immune regulation and cytolysis, forming dimers and trimers.
Applications Reported:
Used for intracellular staining in flow cytometric analysis, cytokine neutralization, and ELISA. FITC conjugated TN3-19.12 is recommended for flow cytometry. Functional Grade purified TN3-19.12 (Product #00-4956-58) can be used ≤20 µg/mL. Titrate antibody for optimal performance.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine of the TNF superfamily, mainly secreted by macrophages. It binds TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors and regulates immune cells, proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha forms multimers (2–5 units) and shows a 17 kDa band in SDS-PAGE under non-reducing conditions. Knockout studies indicate neuroprotective functions and tumor necrosis effects. It is involved in immune responses against infections and tumor cytolysis, acting synergistically with interferon-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.