Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), FITC, eBioscience
인간 TNF-α를 인식하는 중화능 단클론 항체로, FITC 형광 표지되어 있습니다. 유세포 분석 및 세포 내 염색에 적합하며, 488 nm/520 nm 파장에서 검출됩니다. 4°C 암조건에서 보관하며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 27 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb11 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10718392 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 650
- eFluor 450
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays a key role in immune regulation. It exists as dimers and trimers and also as a 26 kDa membrane-bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported: MAb11 has been reported for use in intracellular flow cytometric analysis.
Applications Tested: Pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per test (final volume 100 µL). Cell number may vary from 10⁵ to 10⁸ cells/test.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
TNF alpha is a multifunctional proinflammatory cytokine of the TNF superfamily. Secreted mainly by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. It regulates immune responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha forms multimers and shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Knockout studies indicate neuroprotective functions and tumor necrosis activity. It also plays roles in immune responses to infections and tumor cytolysis, often synergistic with interferon-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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