Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), FITC, eBioscience
마우스 IFN-γ를 인식하는 중화용 단클론 항체로, FITC로 표지되어 흐름세포분석에 최적화됨. 0.5 µg/test의 권장 사용량으로 높은 특이성과 재현성을 제공. 488 nm 레이저에 적합하며, 액상 형태로 4°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 publications |
| Immunohistochemistry (IHC) | - | 4 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 268 publications |
| ELISA | - | 8 publications |
| Neutralization (Neu) | - | 1 publication |
| Functional Assay | - | 1 publication |
| Miscellaneous (PubMed) | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | XMG1.2 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465411 |
Additional Formats Available
Unconjugated, Alexa Fluor 488, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 615, Brilliant UV 661, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 786, eFluor 450, eFluor 660, Functional Grade, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, Custom conjugation available.
Product Specific Information
Description:
The XMG1.2 antibody reacts with mouse interferon (IFN) gamma, a 20 kDa cytokine produced by activated T, B, and NK cells with antiviral and antiparasitic functions. It is a neutralizing antibody that modulates immune responses by inhibiting cell proliferation in synergy with TNF alpha. Glycosylation does not affect IFN gamma activity.
Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested for intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Excitation/Emission: 488 nm / 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens, mitogens, and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. IFN gamma exhibits species-specific activity; human IFN gamma does not cross-react with mouse. Functions include antiviral and antiproliferative effects, MHC induction, macrophage activation, and enhanced immunoglobulin secretion by B cells.
Activation occurs via IFN gamma receptor I and II, triggering the JAK-STAT pathway. Expression is upregulated by IL-2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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