Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 615, Brilliant UV 661, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 786, eFluor 450, eFluor 660, Functional Grade, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, Custom conjugation available.

Product Specific Information

Description:
The XMG1.2 antibody reacts with mouse interferon (IFN) gamma, a 20 kDa cytokine produced by activated T, B, and NK cells with antiviral and antiparasitic functions. It is a neutralizing antibody that modulates immune responses by inhibiting cell proliferation in synergy with TNF alpha. Glycosylation does not affect IFN gamma activity.

Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested for intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. Recommended ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.

Excitation/Emission: 488 nm / 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IFN gamma (Interferon gamma, Type II interferon):
A macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens, mitogens, and cytokines. It is a dimeric glycoprotein (~45 kDa) composed of two 146 amino acid subunits. IFN gamma exhibits species-specific activity; human IFN gamma does not cross-react with mouse. Functions include antiviral and antiproliferative effects, MHC induction, macrophage activation, and enhanced immunoglobulin secretion by B cells.

Activation occurs via IFN gamma receptor I and II, triggering the JAK-STAT pathway. Expression is upregulated by IL-2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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