Takara Campylobacter PCR detection and typing kit

Conventional culture identification of Campylobacter species takes weeks, but the Campylobacter (cdt gene) PCR Detection and Typing Kit specifically and rapidly identifies Campylobacter jejuni (C. jejuni), Campylobacter coli (C. coli), and Campylobacter fetus (C. fetus) within hours. The kit uses PCR to detect the B subunit (cdtB) and C subunit (cdtC) of the cytolethal distending toxin (cdt) genes. Ex Taq HS version, cdtB and cdtC primer mixes, and positive controls are included.

Conventional culture identification of Campylobacter species takes weeks, but the Campylobacter (cdt gene) PCR Detection and Typing Kit specifically and rapidly identifies Campylobacter jejuni (C. jejuni), Campylobacter coli (C. coli), and Campylobacter fetus (C. fetus) within hours. The kit uses PCR to detect the B subunit (cdtB) and C subunit (cdtC) of the cytolethal distending toxin (cdt) genes. Ex Taq HS version, cdtB and cdtC primer mixes, and positive controls are included.

In the Campylobacter species, there are occasional strains with deletions or mutations on their cdtB gene or cdtC gene. In order to increase the detection accuracy of this kit, detection of both cdtB and cdtC genes is performed. Takara Bio’s Ex Taq HS enzyme decreases mispriming during the preparation of reaction solutions and non-specific amplifications derived from primer dimers.

This kit was designed by Takara Bio in collaboration with Osaka Prefecture University and Fuso Pharmaceutical Industries, Ltd.

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