Thermo Fisher Scientific CD135 (Flt3) Monoclonal Antibody (BV10A4H2), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 5 publications

Product Specifications

Additional Formats

• PE format
• Request custom conjugation

Product Specific Information

The BV10A4H2 monoclonal antibody reacts with human CD135 (Flk2/Flt3), a 135–150 kDa tyrosine kinase receptor expressed on multipotential progenitor cells including primitive B cell and myelomonocytic progenitors in fetal liver and adult bone marrow.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on REH cell line
• Recommended Use: 5 µL (0.5 µg) per 100 µL test volume
• Cell Density: 10⁵–10⁸ cells/test (empirically determined)
• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

FLT3 is a tyrosine protein kinase belonging to the growth factor receptor family. Upon ligand binding, it dimerizes and autophosphorylates, activating downstream pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Constitutive activation mutations are implicated in acute myeloid and lymphoblastic leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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