Thermo Fisher Scientific 4EBP1 Monoclonal Antibody (E.992.6)
4EBP1 단백질을 인식하는 Rabbit Monoclonal Antibody (Clone E.992.6). Western blot, IHC, ICC/IF, Flow cytometry, IP 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 액상 형태, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,400 |
| Immunocytochemistry (ICC/IF) | 1:1,600 |
| Flow Cytometry (Flow) | 1:1,600 |
| Immunoprecipitation (IP) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.992.6 |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser112 of human 4E-BP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 169 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10980293 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5′ end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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