Thermo Fisher Scientific TSC2 Monoclonal Antibody (T.309.0)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

T.309.0

Immunogen

Synthetic peptide from the amino terminus of human tuberin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TSC2,uniProtId:P49815-1,ncbiNodeId:9606,antigenRange:1,antigenLength:1807,antigenImageFileName:MA5-15004_TSC2_P49815-1_Rabbit.svg,antigenImageFileNamePDP:MA5-15004_TSC2_P49815-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

270 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10987349

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

TSC2 (Tuberin, Tuberous sclerosis complex, TSC Complex Subunit 2), is implicated as a tumor suppressor and may function in vesicular transport, play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. TSC2 associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Phosphorylation of tuberin on Ser 939 and Thr 1462 regulates its interaction with hamartin, and is stimulated by various growth factors through the phosphoinositide 3-kinase/Akt pathway. TSC2 may have a function in vesicular transport, but may also play a role in the regulation of cell growth arrest and in the regulation of transcription mediated by steroid receptors. Interaction between TSC1 and TSC2 may facilitate vesicular docking. TSC2 specifically stimulates the intrinsic GTPase activity of the Ras related protein RAP1A and RAB5, suggesting a possible mechanism for its role in regulating cellular growth. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors and are involved in diseases such as lymphangioleiomyomatosis and TSC2 angiomyolipoma. Alternative splicing results in transcript variants of three different isoforms of TSC2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.