Thermo Fisher Scientific AKT Pan Monoclonal Antibody (E.32.10)
AKT Pan 단백질에 특이적인 Rabbit Monoclonal Antibody로, Western blot, IHC, ICC, Flow Cytometry, IP, ChIP 등 다양한 응용에 적합. 인간, 생쥐, 원숭이, 랫트 반응성. 고순도 친화 크로마토그래피 정제, 액상 형태로 안정적 보관 가능.
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Thermo Fisher Scientific AKT Pan Monoclonal Antibody (E.32.10)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | 1:100 | - |
| Immunoprecipitation (IP) | 1:100 | - |
| ChIP assay (ChIP) | 1–3 µL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | E.32.10 |
| Immunogen | Synthetic peptide at the carboxy-terminal sequence of mouse Akt |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 19 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10983774 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other related proteins.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in regulating metabolism, cell survival, and growth by phosphorylating multiple proteins.
Activated by insulin, PI3K, IGF1, and other growth factors, Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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