Thermo Fisher Scientific AKT Pan Monoclonal Antibody (E.32.10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:100

-

Immunoprecipitation (IP)

1:100

-

ChIP assay (ChIP)

1-3 µL

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.32.10

Immunogen

Synthetic peptide at the carboxy-terminal sequence of mouse Akt if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT Pan,uniProtId:P31750-1,ncbiNodeId:10090,antigenRange:480,antigenLength:480,antigenImageFileName:MA5-14999_AKT_Pan_P31750-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14999_AKT_Pan_P31750-1_Rabbit_PDP.jpeg,sortOrder:4},{targetFamily:AKT Pan,uniProtId:Q60823-1,ncbiNodeId:10090,antigenRange:481,antigenLength:481,antigenImageFileName:MA5-14999_AKT_Pan_Q60823-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14999_AKT_Pan_Q60823-1_Rabbit_PDP.jpeg,sortOrder:5},{targetFamily:AKT Pan,uniProtId:Q9WUA6-1,ncbiNodeId:10090,antigenRange:479,antigenLength:479,antigenImageFileName:MA5-14999_AKT_Pan_Q9WUA6-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14999_AKT_Pan_Q9WUA6-1_Rabbit_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

19 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10983774

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other related proteins.

Target Information

AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.