Thermo Fisher Scientific VDR Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-711 detects vitamin D receptor (VDR) from human, mouse, and rat tissues.
It has been successfully used in Western blot, immunoprecipitation, and gel shift assays.
By Western blot, this antibody detects an approximately 53 kDa protein representing VDR in COS-7 cells transfected with the human gene.
PA1-711 supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).

The immunizing peptide corresponds to amino acid residues 395–413 from human VDR.
Recombinant human VDR (Cat. # RP-710) and human RXR beta (Cat. # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation, and gel shift assays.

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to DNA sequences following activation by the appropriate hormone.
The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors, which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors.

Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation.
VDR has been found in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system.
Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression occurs when VDR binds within the IL-2 enhancer.
Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 interferes with assembly of NFATp/Fos/Jun/DNA complex and subsequent IL-2 gene transcription.

The corresponding gene for the vitamin D receptor is NR1I1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.