Thermo Fisher Scientific Dynamin 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 14 publications 14 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dynamin 1,uniProtId:P21575-1,ncbiNodeId:10114,antigenRange:633-647,antigenLength:864,antigenImageFileName:PA1-660_Dynamin_1_P21575-1_Rabbit.svg,antigenImageFileNamePDP:PA1-660_Dynamin_1_P21575-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325845

Product Specific Information

PA1-660 detects dynamin I (Dyn1) from rat tissues.

PA1-660 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn1 from rat whole brain extract.

PA1-660 immunizing peptide corresponds to amino acid residues 633-647 from rat Dyn1. This sequence is completely conserved between rat, mouse, and human Dyn1. PA1-660 immunizing peptide (Cat. # PEP-042) is available for use in neutralization and control experiments.

Target Information

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin`s involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.