Thermo Fisher Scientific 7-Ketocholesterol Monoclonal Antibody (3F7), PE

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

3F7

Immunogen

Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Conjugate

PE PE PE

View additional formats

• Unconjugated
• APC
• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

47.35mM phosphate/2.15mM MES, pH 7.4, with 1.033mM EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932254

Product Specific Information

A 1:1,000 dilution of MA5-45800 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for 7-ketocholesterol modified proteins.

Target Information

7-Ketocholesterol is a major oxidation product of cholesterol found in human atherosclerotic plaque and is more atherogenic than cholesterol in some animal studies. 7-Ketocholesterol can inhibit cholesterol 7alpha-hydroxylase, the rate-limiting step in bile acid biosynthesis, as well as strongly inhibiting HMG-CoA reductase, the rate-limiting enzyme in cholesterol biosynthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.