Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (6H6), APC
Malondialdehyde 단백질 결합체를 특이적으로 인식하는 APC-conjugated 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 사용 가능. 단백질 산화 스트레스 연구에 적합. 보존제 무첨가, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6H6 |
| Immunogen | Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.46 mM phosphate / 2.48 mM MES, pH 7.4, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932255 |
Additional Formats Available
- Unconjugated (MA5-27559)
- FITC (MA5-45802)
- PE (MA5-45804)
- PerCP (MA5-45803)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45801 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Malondialdehyde-conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Target Information
Malondialdehyde (CH₂(CHO)₂) is an organic compound and a byproduct of lipid metabolism. It is a highly reactive electrophile species that can cause toxic stress in cells, forming covalent protein adducts known as advanced lipoxidation end products (ALE). It also forms mutagenic DNA adducts with deoxyadenosine and deoxyguanosine. Malondialdehyde is present in many foods and found at high levels in rancid items.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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