Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Brilliant Ultra Violet 737, eBioscience
Flow cytometry용 Rat IgG1 kappa 동종 대조 항체로, Brilliant Ultra Violet 737 형광체 결합. 실험 항체의 비특이적 결합 평가에 적합. 자외선(355 nm) 레이저 장비용. 4°C 암소 보관 권장.
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Applications
Tested Dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | Brilliant Ultra Violet™ 737 |
| Excitation / Emission Max | 350 / 740 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896005 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 805
- Brilliant Violet 421, 480, 605, 650, 711, 786
- eFluor 450, 506, 570, 615, 660
- FITC
- Functional Grade
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available upon request
Product Specific Information
The monoclonal rat IgG1 kappa is useful as an isotype control immunoglobulin.
This eBRG1 antibody is reported for use in flow cytometric analysis and has been tested on mouse splenocytes and normal human peripheral blood cells. It should be used at the same concentration as the experimental antibody.
Brilliant Ultra Violet™ 737 (BUV737) is a tandem dye emitting at 732 nm, designed for use with cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), it is recommended to use:
- Super Bright Complete Staining Buffer (Product # SB-4401)
- Brilliant Stain Buffer (Product # 00-4409)
These buffers minimize non-specific polymer interactions.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Methanol Compatibility: Compatible with short-term methanol fixation; avoid storage in methanol-containing buffers for longer than one hour.
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used as a negative control in research applications. It does not bind to most mammalian antigens and is used in immunohistochemistry, flow cytometry, and western blotting. Rat IgG1 antibodies are produced in response to antigens and can be purified from serum or hybridoma culture supernatants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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