Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), Biotin, Functional Grade, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (TN3-19.12), Biotin, Functional Grade

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit TNF-alpha, but not with human TNF-alpha. It is a neutralizing antibody. Mouse TNF-alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells, and NK cells. A 26 kDa membrane-bound form also exists. TNF-alpha plays an important role in immune regulation and can form dimers and trimers.

Applications Reported:
The Functional Grade Biotin anti-mouse TNF alpha antibody (clone TN3-19) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA), which enhances cytokine detection sensitivity up to 1000-fold by extending in vivo half-lives.

For detailed methodology, refer to:
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology, Unit 6.28. John Wiley and Sons, New York.

The TN3-19.12 antibody is suitable for intracellular staining, cytokine neutralization, and ELISA.

• Fluorochrome-conjugated TN3-19.12 is recommended for flow cytometry.
• Functional Grade purified TN3-19.12 (Product # 16-7423-81) is recommended for functional assays.
• For ELISPOT capture, use clone 1F3F3D4 (Product # 16-7325).

Applications Tested:
The Functional Grade Biotin TN3-19 antibody was tested by LAL assay to confirm endotoxin-free status and validated as a detection antibody in sandwich ELISA for mouse TNF-alpha, using polyclonal anti-mouse TNF alpha (Product # 14-7341-85) for capture and recombinant TNF-alpha (Product # 14-8321-62) as the standard.

Recommended ELISA detection concentration: 0.5–2 µg/mL
Standard curve range: 2000 pg/mL – 15 pg/mL

Endotoxin: Less than 0.05 ng/µg antibody (LAL assay)
Storage and Handling: Use in a sterile environment.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine from the TNF superfamily. Secreted mainly by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF alpha regulates immune responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. It appears as a 17 kDa band under non-reducing SDS-PAGE and exists as multimers of two to five units.

Knockout studies in mice indicate TNF alpha has neuroprotective functions and induces tumor necrosis in tumor-bearing mice. It plays key roles in immune defense against infections and tumor cell cytolysis, synergistic with interferon-gamma. TNF alpha is implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.