Thermo Fisher Scientific IHOG Monoclonal Antibody (3G8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Fruit fly, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3G8

Immunogen

Purified recombinant fragment of human IHOG expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10982020

Product Specific Information

MA5-15648 targets IHOG in indirect ELISA, WB applications and shows reactivity with Drosophila samples.

The MA5-15648 immunogen is purified recombinant fragment of human IHOG expressed in E. Coli.

MA5-15648 detects IHOG which has a predicted molecular weight of approximately 98kDa.

Target Information

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type 1 membrane protein shown here to bind and to mediate response to the active Hedgehog (Hh) protein signal. ihog mutations produce defects characteristic of Hh signaling loss in embryos and imaginal discs, and epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc). The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc. Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, thus identifying an evolutionarily conserved family of membrane proteins that function in Hh signal response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.