Thermo Fisher Scientific IHOG Monoclonal Antibody (3G8)

Applications and Tested Dilution

Product Specifications

Product Specific Information

MA5-15648 targets IHOG in indirect ELISA and WB applications, showing reactivity with Drosophila samples.
The immunogen is a purified recombinant fragment of human IHOG expressed in E. coli.
MA5-15648 detects IHOG, which has a predicted molecular weight of approximately 98 kDa.

Target Information

The ihog gene (interference hedgehog), identified by RNA interference in Drosophila cultured cells, encodes a type I membrane protein that binds and mediates response to the active Hedgehog (Hh) protein signal.
Mutations in ihog produce defects characteristic of Hh signaling loss in embryos and imaginal discs. Epistasis analysis places ihog action at or upstream of the negatively acting receptor component, Patched (Ptc).

The first of two extracellular fibronectin type III (FNIII) domains of the Ihog protein mediates a specific interaction with Hh protein in vitro, but the second FNIII domain is additionally required for in vivo signaling activity and for Ihog-enhanced binding of Hh protein to cells coexpressing Ptc.

Other members of the Ihog family, including Drosophila Boi and mammalian CDO and BOC, also interact with Hh ligands via a specific FNIII domain, identifying an evolutionarily conserved family of membrane proteins involved in Hh signal response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.