Thermo Fisher Scientific CD100 (SEMA4D) Monoclonal Antibody (BMA12 (BMA-12)), eBioscience
Thermo Fisher Scientific CD100 (SEMA4D) Monoclonal Antibody는 mouse CD100/Sema4D를 인식하는 rat IgG2a 항체입니다. Flow cytometry, IHC, IP, functional assay에 사용 가능하며, 0.5 mg/mL 농도의 액상 제품입니다. B세포 활성화 및 CD72 상호작용 연구에 적합합니다.
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- 14-1001-xx (2개 옵션)
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Flow Cytometry (Flow) | 0.25 µg/test |
| Immunoprecipitation (IP) | Assay-Dependent |
| Functional Assay (Functional) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BMA12 (BMA-12) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864950 |
Additional Formats
Product Specific Information
Description:
The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D. Sema4D/CD100 is a 150 kDa transmembrane protein expressed constitutively on T cells and involved in activation of B cells through interaction with CD72. This interaction reduces the negative signaling normally mediated by CD72. CD100 is strongly expressed on resting T cells and weakly on B cells, with expression upregulated upon activation. Antigen expression has been reported in spleen, thymus, and lymph nodes.
Applications Reported:
The BMA12 (BMA-12) antibody has been reported for use in:
- Flow cytometric analysis
- Immunoprecipitation
- Immunohistochemical staining
- Neutralization of mouse CD100 (use Functional Grade purified BMA12, Product #16-1001 for functional assays)
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.25 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.
Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Semaphorins are neuronal chemorepellents guiding neurons during development. CD100 (Semaphorin 4D), a 150 kDa homodimer, induces B cell aggregation and enhances viability in vitro. It modulates CD40-CD40L signaling by promoting B cell survival and reducing CD23 expression. CD72 acts as a receptor for CD100, regulating B cell signaling. CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and at higher levels on activated T and B cells.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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