Thermo Fisher Scientific CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), eBioscience
인체 CD100(SEMA4D)을 인식하는 eBio133-1C6 단클론 항체로, Flow Cytometry, ELISA, Immunoprecipitation에 적합. 혈액세포 분석에 사용 가능하며, 높은 순도(>90%)와 낮은 응집도(<10%)를 보유. 연구용으로만 사용.
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Thermo Fisher Scientific CD100 (SEMA4D) Monoclonal Antibody (eBio133-1C6 (133-1C6)), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.5 µg/test | - |
| ELISA | Assay-Dependent | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio133-1C6 (133-1C6) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864954 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D.
CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. It acts as a costimulatory molecule in adaptive immune responses by switching off negative signaling from CD72.
Another ligand of CD100 is Plexin-B1. High expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients. Expression occurs in both hematopoietic and non-hematopoietic cells.
CD100 is expressed on resting T cells, NK cells, and B cells, and can also exist as a soluble form.
Applications Reported: Flow cytometric analysis, immunoprecipitation, and ELISA.
Applications Tested: Tested by flow cytometric analysis of human peripheral blood cells. Recommended ≤0.5 µg/test (100 µL reaction volume). Cell number: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.
Target Information
Semaphorins are neuronal chemorepellants guiding neurons during nervous system development.
CD100 (Semaphorin 4D), a 150 kDa homodimer, induces B cell aggregation and enhances viability in vitro.
It modulates CD40-CD40L B cell signaling by increasing aggregation/survival and downregulating CD23 expression.
CD72 serves as a lymphocyte receptor for CD100, regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, with higher density on activated T and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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