Thermo Fisher Scientific SEMA4A Monoclonal Antibody (KL-1 (aka SK31)), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Rat

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

KL-1 (aka SK31)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10547286

Product Specific Information

Description: The KL-1 (aka SK31) antibody reacts with mouse Sema4A, a class IV semaphorin originally identified as SemB. Sema4A is structurally similar to Sema4D/CD100. Sema4A is reported to be expressed by dendritic cells, B cells and activated T cells. Although published data suggested that Sema4A binds to Tim-2, a T cell surface receptor, it remains to be definitively shown that these two molecules have a receptor/ligand relation. It is suggested that Sema4a can provide costimulatory signals for T cells.

This KL-1 antibody works in immunoblotting but requires non-reducing conditions.

Applications Reported: This KL-1 (aka SK31) antibody has been reported for use in immunoblotting (WB).

Applications Tested: The KL-1 (aka SK31) antibody has been tested by immunoblot analysis of mouse Sema4A transfected cells under nonreducing conditions. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SEMA4A is a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in guidance of axonal migration during neuronal development and in immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.