
Thermo Fisher Scientific IgM Monoclonal Antibody (HIS40), eBioscience
Rat IgM µ heavy chain을 인식하는 HIS40 단클론 항체로, Flow Cytometry 및 IHC 분석에 적합합니다. 고순도(>90%)로 정제된 액상형 제품이며, 다양한 형광체 결합 형식(FITC, PE 등)으로 제공 가능합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific IgM Monoclonal Antibody (HIS40), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS40 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864948 |
Additional Formats
Product Specific Information
The HIS40 monoclonal antibody reacts with the µ heavy chain of rat IgM. It does not react with other classes of rat immunoglobulin including IgD, IgG, or IgA.
IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral B cells.
Fluorochrome-conjugated HIS40 is used as a detection secondary antibody for rat IgM primary antibodies.
Applications Reported:
- Flow cytometric analysis
- Immunohistochemical staining
Applications Tested:
The HIS40 antibody has been tested by flow cytometric analysis of rat splenocytes and can be used at ≤0.5 µg per test (100 µL final volume).
Cell numbers may vary (10⁵–10⁸ cells/test) and should be empirically determined.
It is recommended to titrate the antibody for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Rat IgM is an immunoglobulin M isotype antibody produced by B cells in rats. It plays a crucial role in the immune system by recognizing and neutralizing antigens such as bacteria and viruses.
Rat IgM is widely used in immunology, microbiology, and biotechnology research as a control reagent or diagnostic tool for detecting infectious diseases and autoimmune disorders.
Production and purification techniques include hybridoma technology, protein A/G chromatography, and affinity chromatography.
Rat IgM contributes to understanding immune mechanisms and developing new therapeutic strategies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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