Thermo Fisher Scientific RET Recombinant Rabbit Monoclonal Antibody (SN20-28)
RET 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션으로 제조되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SN20-28 |
| Immunogen | Synthetic peptide within Human RET (aa 1,065–1,114) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809623 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal production clones.
Advantages include:
- Enhanced specificity and sensitivity
- Consistent lot-to-lot performance
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The receptor tyrosine kinase RET belongs to the cadherin superfamily and is crucial for neural crest development. RET can undergo oncogenic activation through cytogenetic rearrangements and mediates cell growth and differentiation signals.
Mutations in the RET gene are associated with:
- Multiple endocrine neoplasia type IIA and IIB
- Hirschsprung disease
- Medullary thyroid carcinoma
Two transcript variants encoding different isoforms have been identified, with additional variants described but not fully validated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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