Thermo Fisher Scientific Phospho-MSK1 (Ser212) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RPS6KA5(Accession O75582), corresponding to amino acid residues around phosphorylated Ser212. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MSK1,uniProtId:O75582-1,ncbiNodeId:9606,antigenRange:212,antigenLength:802,antigenImageFileName:PA5-104711_MSK1_O75582-1_Rabbit.svg,antigenImageFileNamePDP:PA5-104711_MSK1_O75582-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816184

Product Specific Information

Antibody detects endogenous levels of MSK1 only when phosphorylated at Serine 212.

Target Information

RPS6KA5 (MSK1) is a serine/threonine protein kinase that is essential for the regulation of various cellular processes. RPS6KA5 is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at Ser-276 during oxidative stress. In erythropoietin-stimulated cells, is necessary for the Ser-727 phosphorylation of STAT3 and regulation of its transcriptional potential.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.