Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Biotin, eBioscience
마우스 CD16/CD32 항원을 인식하는 Rat IgG2a 단일클론 항체로, Biotin으로 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 0.125 µg/test로 사용 가능합니다. 면역세포 표면 Fc 수용체 분석에 적합하며, 4°C 암소 보관이 권장됩니다.
- 카탈로그번호
- 13-0161-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Biotin, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 14 publications |
| Affinity Purification (AP) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fruit fly, Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 93 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466377 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Brilliant UV 496
- Brilliant UV 661
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Red 710
- NovaFluor Red 755
- NovaFluor Yellow 690
- PE
- PE-Cyanine7
- PerCP-Cyanine5.5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
Description:
The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.125 µg per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal performance.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD16 is a 50–65 kDa cell surface molecule that exists in two forms — a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol-linked form expressed by granulocytes.
It functions as a low affinity receptor for IgG (FcR III) and mediates ADCC (antibody-dependent cell-mediated cytotoxicity) by NK cells.
CD32 is a 40 kDa glycoprotein acting as a low affinity receptor for IgG (Fc gamma RII), mediating endocytosis, secretion activation, cytotoxicity, and immunomodulation. It is expressed by B cells, monocytes, granulocytes, and platelets.
CD32 participates in phagocytosis of immune complexes and regulation of antibody production by B cells. Variations in the CD32 gene may increase susceptibility to systemic lupus erythematosus (SLE).
Several transcript variants encoding different isoforms of CD32 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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