Thermo Fisher Scientific CD16/CD32 Monoclonal Antibody (93), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• Brilliant UV 496
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 690
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Custom conjugation available upon request

Product Specific Information

Description:
The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.125 µg per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50–65 kDa cell surface molecule that exists in two forms — a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol-linked form expressed by granulocytes.
It functions as a low affinity receptor for IgG (FcR III) and mediates ADCC (antibody-dependent cell-mediated cytotoxicity) by NK cells.

CD32 is a 40 kDa glycoprotein acting as a low affinity receptor for IgG (Fc gamma RII), mediating endocytosis, secretion activation, cytotoxicity, and immunomodulation. It is expressed by B cells, monocytes, granulocytes, and platelets.
CD32 participates in phagocytosis of immune complexes and regulation of antibody production by B cells. Variations in the CD32 gene may increase susceptibility to systemic lupus erythematosus (SLE).
Several transcript variants encoding different isoforms of CD32 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.