Thermo Fisher Scientific CD14 Monoclonal Antibody (Sa2-8), Biotin, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (Sa2-8), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• APC
• FITC
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53–55 kDa GPI-linked glycoprotein. CD14 is a receptor for LPS-LBP complexes and associates with Toll-Like Receptor 4 (TLR4), participating in bacterial LPS signaling. In mouse, CD14 is expressed on macrophages and can also be found in serum in secreted form. Sa2-8 exhibits weak antagonistic activity in NF-κB activation or TNF-α production under LPS stimulation.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells at ≤0.5 µg/test (100 µL final volume). Cell number can range from 10⁵–10⁸ cells/test. Titrate for optimal assay performance.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and is released into serum in soluble form. CD14 binds LPS via LBP, discriminates structural variations, and aids in neutralization of serum lipopolysaccharides. It also binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing produces multiple transcript variants encoding the same isoform. CD14 dysfunction is associated with diseases such as Mycobacterium chelonae infection and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.