Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 480
• eFluor 450
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa. CD16 includes two similar genes, CD16A (FcγRIIIA) and CD16B (FcγRIIIB).

• CD16A: expressed in macrophages and NK cells (polypeptide-anchored form)
• CD16B: expressed in neutrophils (GPI-anchored form)

Two polymorphisms, NA-1 and NA-2, are known for CD16B. NA-2 homozygotes show reduced phagocytic capacity compared with NA-1. CD16 binds IgG immune complexes, showing preferential binding to IgG1 and IgG3, minimal to IgG2 and IgG4. Upon IgG binding, CD16 initiates signaling cascades leading to ADCC, phagocytosis, degranulation, and proliferation.

Applications Reported:
This antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Validated by flow cytometry on normal human peripheral blood cells. Recommended usage ≤ 0.125 µg per test (100 µL final volume). Optimal titration should be determined experimentally.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of IgG and participates in immune complex clearance, cellular-mediated cytotoxicity, and viral infection enhancement.

• Gene: FCGR3A (chromosome 1)
• Related gene: FCGR3B (similar sequence, expressed in PMN)
• FCGR3A: membrane glycoprotein anchored via transmembrane peptide
• FCGR3B: anchored via phosphatidylinositol linkage

Mutations in FCGR3A are associated with immunodeficiency 20, recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternative splicing produces different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.