Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Alexa Fluor 532, eBioscience
마우스 CD4 분자에 특이적인 RM4-5 단클론 항체로 Alexa Fluor 532 형광체가 결합된 제품입니다. Flow cytometry 및 면역염색에 적합하며, 532 nm 녹색 레이저로 여기되어 561 nm에서 방출됩니다. 0.25 µg/test로 사용 가능하며, 4°C 암소 보관을 권장합니다.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Alexa Fluor 532, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 11 publications |
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunohistochemistry (Frozen) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 4 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 124 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 532, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RM4-5 |
| Conjugate | Alexa Fluor™ 532 |
| Excitation / Emission Max | 534 / 553 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11219484 |
Available Formats
- Unconjugated
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615
- FITC
- NovaFluor Blue / Yellow series
- Pacific Orange
- PE and variants (PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610)
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright series (436, 600, 645, 702, 780)
- Request custom conjugation
Product Specific Information
The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T-cell subpopulations, and dendritic cells. CD4 binds MHC class II on antigen-presenting cells and plays an essential role in T-cell development and function.
Binding of RM4-5 is blocked by GK1.5.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse thymocytes and splenocytes (≤ 0.25 µg/test, 100 µL final volume)
- Recommended to titrate for optimal performance
Alexa Fluor® 532 is excited with the green laser (532 nm) and emits at 561 nm.
Not compatible with yellow-green laser (561 nm).
Recommended filter: 560/14 band pass.
Excitation: 532 nm
Emission: 561 nm
Laser: Green laser
Target Information
CD4 antigen is involved in MHC class II recognition and functions as a co-receptor for HIV. It is primarily expressed in T-helper lymphocytes, and also in monocytes, macrophages, and dendritic cells.
Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut. CD4 contributes to early T-cell activation and may mediate neuronal damage in immune-related CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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