
Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 568
Alexa Fluor 568 형광이 결합된 염소 유래 항-닭 IgY (H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry에 적합합니다. 높은 형광 안정성과 감도를 제공하며, 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Concentration |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 568 |
| Excitation / Emission Max | 579 / 603 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2534098 |
| Target | IgY |
| Antibody Form | Whole Antibody |
Alternative Conjugates Available
- Alexa Fluor 488
- Alexa Fluor 546
- Alexa Fluor 555
- Alexa Fluor 594
- Alexa Fluor 647
- AP
- Biotin
- FITC
- HRP
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
Alexa Fluor dyes are highly trusted fluorescent dyes known for brightness and stability.
Invitrogen™ Alexa Fluor 568 dye emits bright orange/red fluorescence, optimally excited by the 568 nm laser line. It remains pH-insensitive over a wide range, ensuring stable signal generation in imaging and flow cytometry.
With high quantum yield and photostability, Alexa Fluor 568 enables sensitive detection of low-abundance targets.
Typically, 2–8 fluorophore molecules are conjugated per IgG molecule; the exact labeling degree is provided in the product’s certificate of analysis.
Usage Note:
Before use, briefly centrifuge the conjugate solution and use only the supernatant to remove any aggregates. Optimal antibody dilution should be determined empirically; 1–10 µg/mL is generally suitable for IHC and flow cytometry.
Shipping: Product ships at room temperature.
Target Information
Anti-Chicken secondary antibodies are affinity-purified reagents specific for chicken immunoglobulins.
They are widely used for detection, sorting, or purification of chicken antibodies.
Secondary antibodies increase assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically produced by immunizing the host with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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