Thermo Fisher Scientific ID4 Monoclonal Antibody (OTI2G10), TrueMAB
ID4 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western Blot에 최적화되어 있습니다. HEK293T 세포에서 생산된 full-length human ID4 단백질을 면역원으로 사용하였으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G10 |
| Immunogen | Full length human recombinant protein of human ID4 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional round of desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
ID4 is a transcription factor containing a basic helix-loop-helix (bHLH) motif that regulates expression of tissue-specific genes in various mammalian and insect systems.
Its DNA-binding activity depends on formation of homo- and/or heterodimers.
Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, contain the HLH dimerization domain but lack the DNA-binding basic domain.
As a result, Id proteins inhibit DNA binding and transcriptional activation by heterodimerization with bHLH proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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