Thermo Fisher Scientific AQP0 Polyclonal Antibody
Rabbit polyclonal antibody against human AQP0. Validated for WB and IHC-P applications. Lyophilized form, reconstitutable in sterile water. Shares 95% identity with rat, mouse, and guinea pig sequences. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000 |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from aa 140–180 of human AQP0 conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at −20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
- The peptide shares 95% identity with rat, mouse, and guinea pig sequences.
- Specificity: AQP0.
Target Information
AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells, functioning both as a water pore and in fiber-fiber adhesion, crucial for lens structure and organization.
It contains an additional pore constriction unique among aquaporins, possibly responsible for pore gating.
The closed AQP0 pore accommodates only three water molecules, spaced too far apart to form hydrogen bonds.
The C-terminal domain undergoes extensive post-translational modifications, including truncations during lens aging, potentially contributing to cataract development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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