Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Ultra Violet 661

Applications and Tested Dilution

• Flow Cytometry (Flow): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The monoclonal mouse IgG2b kappa is useful as an isotype control immunoglobulin.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on normal human peripheral blood and mouse splenocytes; use at same concentration as experimental antibody
• Fluorochrome: Brilliant Ultra Violet™ 661 (BUV661), emits at 660 nm, optimized for UV laser (355 nm) detection
• Light Sensitivity: Protect from light; sensitive to photo-induced oxidation
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark
• Recommended Buffers: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions
• Excitation / Emission / Laser: 350 nm / 660 nm / Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

Mouse IgG2b is a subtype of immunoglobulin G produced by B cells in response to antigens. It plays key roles in:

• Opsonization and complement activation
• Antibody-dependent cell-mediated cytotoxicity
• Regulation of immune responses (T cell activation and B cell inhibition)

Widely used in research and diagnostic applications such as ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic research including immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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